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DNMT1 antibody [60B1220.1]

Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Flow cytometry (FACS). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548
Anti-DNMT1 antibody [60B1220.1] used in IHC (Paraffin sections) (IHC-P). GTX48548

Cat. No. GTX48548

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 60B1220.1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, MS, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Pig
Package
50 μg ($399)
See all DNMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.5 μg/ml
ICC/IF 1:10
IHC-P 1:50 - 1:500
FACS Assay dependent
IP 1:10 - 1:500
ChIP assay 1:20 - 1:1000
MS Assay dependent
IHC (Free Floating) 1:500
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548

GTX48548 WB Image

WB analysis of HeLa cell lysate using GTX48548 DNMT1 antibody [60B1220.1].

Anti-DNMT1 antibody [60B1220.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48548

GTX48548 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-DNMT1 antibody [60B1220.1] used in Flow cytometry (FACS). GTX48548

GTX48548 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA

Anti-DNMT1 antibody [60B1220.1] used in Western Blot (WB). GTX48548

GTX48548 WB Image

WB analysis of 2102EP cells / human DNMT1 recombinant protein / NIH3T3 cells / D3 cells in the (A)/(C)/(E)/(G) absence and (B)/(D)/(F)/(H) presence of immunizing peptide using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 5 μg/ml for cells / 0.1μg/ml for recombinant protein

Anti-DNMT1 antibody [60B1220.1] used in IHC (Paraffin sections) (IHC-P). GTX48548

GTX48548 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma tissue using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399