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DNMT1 antibody

KO/KD
Anti-DNMT1 antibody used in Western Blot (WB). GTX64361
Anti-DNMT1 antibody used in Immunoprecipitation (IP). GTX64361
Anti-DNMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64361
Anti-DNMT1 antibody used in Western Blot (WB). GTX64361

Cat. No. GTX64361

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all DNMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody used in Western Blot (WB). GTX64361

GTX64361 WB Image

WB analysis of various sample lysates using GTX64361 DNMT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DNMT1 antibody used in Immunoprecipitation (IP). GTX64361

GTX64361 IP Image

IP analysis of 293T cell lysate using GTX64361 DNMT1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-DNMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64361

GTX64361 ICC/IF Image

ICC/IF analysis of 293T cells using GTX64361 DNMT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DNMT1 antibody used in Western Blot (WB). GTX64361

GTX64361 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX64361 DNMT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399