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DNMT3A antibody

KO/KD Orthogonal
Anti-DNMT3A antibody used in Western Blot (WB). GTX129126
Anti-DNMT3A antibody used in Western Blot (WB). GTX129126
Anti-DNMT3A antibody used in Western Blot (WB). GTX129126
Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129126
Anti-DNMT3A antibody used in Western Blot (WB). GTX129126
Anti-DNMT3A antibody used in Western Blot (WB). GTX129126
Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129126

Cat. No. GTX129126

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNMT3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

293T nuclear , Neuro2A , PC-12 , Rat2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885903

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS

Cellular Localization

Nucleus , Cytoplasm

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody used in Western Blot (WB). GTX129126

GTX129126 WB Image

KO/KD

Wild-type (WT) and DNMT3A knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129126

GTX129126 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:3000.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129126

GTX129126 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129126

GTX129126 IP Image

Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 μg of DNMT3A antibody (GTX129126) or DNMT3A antibody (GTX129125).
Western blot analysis was performed using DNMT3A antibody (GTX129126) diluted at 1:2000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DNMT3A antibody used in Western Blot (WB). GTX129126

GTX129126 WB Image

Orthogonal

DNMT3A antibody detects DNMT3A protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T nuclear extract
5 % SDS-PAGE
DNMT3A antibody (GTX129126) dilution: 1:5000

Anti-DNMT3A antibody used in Western Blot (WB). GTX129126

GTX129126 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3A antibody used in Immunoprecipitation (IP). GTX129126

GTX129126 IP Image

DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments.
IP samples: 293T nuclear extract
A. 40 μg 293T nuclear extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of DNMT3A protein by 4 μg DNMT3A antibody (GTX129126)
5 % SDS-PAGE
The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129126) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169