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DNMT3B antibody

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851
Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851
Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851
Anti-DNMT3B antibody used in Western Blot (WB). GTX22851

Cat. No. GTX22851

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all DNMT3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.0 μg/ml
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3B , MmuIIIB

Cellular Localization

Nucleus

Background

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851

GTX22851 IHC-P Image

IHC-P analysis of human testis tissue using GTX22851 DNMT3B antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:500

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851

GTX22851 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX22851 DNMT3B antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX22851

GTX22851 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22851 DNMT3B antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:500

Anti-DNMT3B antibody used in Western Blot (WB). GTX22851

GTX22851 WB Image

WB analysis of 25 ug of Hela cell lysates using GTX22851 DNMT3B antibody.
Dilution : 1:1000

REFERENCE

Application Reference

Yen YT et al. Nat Commun 2021; 12 (1) : 7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399