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DOCK8 antibody

Anti-DOCK8 antibody used in Western Blot (WB). GTX130251
Anti-DOCK8 antibody used in IHC (Paraffin sections) (IHC-P). GTX130251

Cat. No. GTX130251

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

239 kDa. ( Note )

Positive Control

Raji , NCI-H929

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DOCK8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886223

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dedicator of cytokinesis 8 , HEL-205 , MRD2 , ZIR8

Background

DOCK8 is a member of the DOCK180-related (DOCK1; MIM 601403) protein family (Ruusala and Aspenstrom, 2004 [PubMed 15304341]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DOCK8 antibody used in Western Blot (WB). GTX130251

GTX130251 WB Image

DOCK8 antibody detects DOCK8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK8 antibody (GTX130251) diluted at a dilution of 1:1000.

Anti-DOCK8 antibody used in IHC (Paraffin sections) (IHC-P). GTX130251

GTX130251 IHC-P Image

DOCK8 antibody detects DOCK8 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
DOCK8 antibody (GTX130251) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169