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DOK5 antibody, C-term

Anti-DOK5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89660
Anti-DOK5 antibody, C-term used in Western Blot (WB). GTX89660

Cat. No. GTX89660

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all DOK5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 1-2μg/ml

Note :

IHC-P
tonsil, stains scattered cells in the interfollicular area compatible with mast cells.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89660-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CLHRTETFPAYRSEH, from the C Terminus of the protein sequence according to NP_060901.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

docking protein 5 , C20orf180 , IRS-6 , IRS6

Background

The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-DOK5 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89660

GTX89660 IHC-P Image

IHC-P analysis of human tonsil using GTX89660 DOK5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 1μg/ml

Anti-DOK5 antibody, C-term used in Western Blot (WB). GTX89660

GTX89660 WB Image

WB analysis of HEK293 overexpressing DOK5 (mock transfection in first lane) using GTX89660 DOK5 antibody, C-term.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399