APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , K562
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DR3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952307
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF receptor superfamily member 25 , APO-3 , DDR3 , DR3 , GEF720 , LARD , PLEKHG5 , TNFRSF12 , TR3 , TRAMP , WSL-1 , WSL-LR
Cellular Localization
Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 9: Cell membrane; Single-pass type I membrane protein , Isoform 11: Cell membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105713 IHC-P Image
DR3 antibody detects TNFRSF25 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
DR3 antibody (GTX105713) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105713 WB Image
DR3 antibody detects TNFRSF25 protein by Western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
10 % SDS-PAGE
DR3 antibody (GTX105713) dilution: 1:1000
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REFERENCE
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REVIEW
There are currently no reviews for DR3 antibody (GTX105713). Be the first to share your experience with this product.
