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DSPP antibody

Anti-DSPP antibody used in Western Blot (WB). GTX134428
Anti-DSPP antibody used in IHC (Paraffin sections) (IHC-P). GTX134428
Anti-DSPP antibody used in IHC (Paraffin sections) (IHC-P). GTX134428

Cat. No. GTX134428

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DSPP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human DSPP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887277

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dentin sialophosphoprotein , DFNA39 , DGI1 , DMP3 , DPP , DSP

Background

This gene encodes a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of proteins. The encoded preproprotein is secreted by odontoblasts and proteolytically processed to generate two principal proteins of the dentin extracellular matrix of the tooth, dentin sialoprotein and dentin phosphoprotein. These two protein products may play distinct but related roles in dentin mineralization. Mutations in this gene are associated with dentinogenesis imperfecta and dentin dysplasia. This gene is present in a gene cluster on chromosome 4. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-DSPP antibody used in Western Blot (WB). GTX134428

GTX134428 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DSPP antibody (GTX134428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-DSPP antibody used in IHC (Paraffin sections) (IHC-P). GTX134428

GTX134428 IHC-P Image

DSPP antibody detects secreted DSPP protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DSPP stained by DSPP antibody (GTX134428) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DSPP antibody used in IHC (Paraffin sections) (IHC-P). GTX134428

GTX134428 IHC-P Image

DSPP antibody detects secreted DSPP protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
DSPP stained by DSPP antibody (GTX134428) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169