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DUOX1 antibody [N1N3]

Orthogonal
Anti-DUOX1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119160
Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160
Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160
Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

Cat. No. GTX119160

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DUOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , unboiled A431 , A431 membrane extract

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human DUOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dual oxidase 1 , LNOX1 , NOXEF1 , THOX1

Cellular Localization

Apical cell membrane

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DUOX1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119160

GTX119160 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DUOX1(GTX119160) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

GTX119160 WB Image

Orthogonal

Boiled and unboiled A431 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

GTX119160 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

GTX119160 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28099519

REFERENCE

Application Reference

Mateus Mota et al. Mol Oncol 2021; 15 (4) : 942-956 Merlin deficiency alters the redox management program in breast cancer.
Application : WB
Reactivity : Human
Seimetz M et al. Nat Metab 2020; 2 (6) : 532-546 NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD.
Application : WB
Reactivity : Human
Jafari N et al. PLoS One 2017; 12 (1) : e0170327 CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169