APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-1.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88749-PEP).
Product Note
This antibody is expected to recognise three reported isoforms (NP_570722.2, NP_001028731.1 and NP_001028732.1).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence PLQVSDYSWQQTKT-C, from the N Terminus of the protein sequence according to NP_570722.2; NP_001028731.1; NP_001028732.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dynein axonemal assembly factor 4 , CILD25 , DYX1 , DYX1C1 , DYXC1 , EKN1 , RD
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a tetratricopeptide repeat domain-containing protein. The encoded protein interacts with estrogen receptors and the heat shock proteins, Hsp70 and Hsp90. An homologous protein in rat has been shown to function in neuronal migration in the developing neocortex. A chromosomal translocation involving this gene is associated with a susceptibility to developmental dyslexia. Mutations in this gene are associated with deficits in reading and spelling. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream cell cycle progression 1 (CCPG1) gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX88749 WB Image
WB analysis of human brain (frontal cortex) lysate using GTX88749 DYX1C1 antibody, N-term. Dilution : 0.5μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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