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Dematin antibody

Anti-Dematin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87430
Anti-Dematin antibody used in Western Blot (WB). GTX87430
Anti-Dematin antibody used in IHC (Paraffin sections) (IHC-P). GTX87430

Cat. No. GTX87430

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Dematin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Dematin (356-405).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DMT , DMTN , EPB49 , dematin actin binding protein , Dematin

Cellular Localization

Cytoplasm,Cytoplasm,Cytosol,Cytoplasm,Perinuclear region,Cytoplasm,Cytoskeleton,Cell membrane,Membrane,Endomembrane system,Cell projection

Background

The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Dematin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87430

GTX87430 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX87430 Dematin antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Dematin antibody used in Western Blot (WB). GTX87430

GTX87430 WB Image

WB analysis of 293 cell lysates using GTX87430 Dematin antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Dematin antibody used in IHC (Paraffin sections) (IHC-P). GTX87430

GTX87430 IHC-P Image

IHC-P analysis of human brain tissue using GTX87430 Dematin antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399