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Dengue virus NS1 protein antibody

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103346
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346

Cat. No. GTX103346

Reference 10
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:1000-1:2000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Dengue virus 2-infected BHK-21

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240703

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1

Research Area

DATA IMAGES

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346

GTX103346 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX103346) diluted at 1:2000.

Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103346

GTX103346 ICC/IF Image

GTX103346 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using NS1 (Dengue virus) antibody (GTX103346) at 1:2000 dilution.

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346

GTX103346 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX103346) diluted at 1:1000.

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346

GTX103346 WB Image

The data was published in the journal Sci Rep in 2015. PMID: 26657027

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346

GTX103346 WB Image

The data was published in the journal Sci Rep in 2015. PMID: 26657027

REFERENCE

Application Reference

Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12 : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5) : 606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : WB
Callaway JB et al. PLoS One 2015; 10 (8) : e0136708 Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1β Secretion: A Primary Human Monocyte Model.
Tham HW et al. J Infect Dev Ctries 2015; 9 (12) : 1338-49 Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library.
Application : ICC/IF
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REVIEW

Dengue virus NS1 protein antibody Cat. No. GTX103346

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX103346
Date : Anonymous submitted on 03-May-2021
Score :
Application Tested : WB
Sample : mouse fibroblast L929 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr
Lane Description : Non-infected (mock) and infected (infection) L929 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX103346) diluted at 1:2000.
Submit a Review
Application Reference
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12:926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Xie X et al. Cell Host Microbe 2019; 26 (5):606-622.e8 Dengue NS2A Protein Orchestrates Virus Assembly.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : WB
Callaway JB et al. PLoS One 2015; 10 (8):e0136708 Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1β Secretion: A Primary Human Monocyte Model.
Tham HW et al. J Infect Dev Ctries 2015; 9 (12):1338-49 Protein-protein interactions between A. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cDNA library.
Application : ICC/IF
Hsu AY et al. Sci Rep 2015; 5:17990 Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance.
Application : WB
Xie X et al. Virology 2014; 450-451:250-7 Generation and characterization of mouse monoclonal antibodies against NS4B protein of dengue virus.
Application : WB, ICC/IF
Zou J et al. J Virol 2014; 88 (6):3379-91 Dimerization of Flavivirus NS4B Protein.
Application : WB
Sridharan A et al. J Virol 2013; 87 (21):11648-58 Inhibition of megakaryocyte development in the bone marrow underlies dengue virus-induced thrombocytopenia in humanized mice.
Application : WB
Lin SW et al. J Infect 2012; 64 (3):325-34 Dengue virus nonstructural protein NS1 binds to prothrombin/thrombin and inhibits prothrombin activation.
Application : WB, ELISA
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169