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Desmoglein 3 antibody [5G11]

Anti-Desmoglein 3 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34676

Cat. No. GTX34676

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all Desmoglein 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Product Note

This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human DSG3 extracellular protein fragment

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDHF6 , DSG3 , PVA , desmoglein 3 , Desmoglein 3

Cellular Localization

Cell membrane,Cell junction,Desmosome

Background

This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 3 antibody [5G11] used in IHC (Paraffin sections) (IHC-P). GTX34676

GTX34676 IHC-P Image

IHC-P analysis of human skin tissue using GTX34676 Desmoglein 3 antibody [5G11].

REFERENCE

Clone Reference

Kitagawa N et al. Anat Sci Int 2018; (Epub) Nisin, a food preservative produced by Lactococcus lactis, affects the localization pattern of intermediate filament protein in HaCaT cells.
Kitagawa N et al. Histochem Cell Biol 2014; Inhibition of JNK in HaCaT cells induced tight junction formation with decreased expression of cytokeratin 5, cytokeratin 17 and desmoglein 3.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529