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Desmoglein 3 antibody [5H10]

Anti-Desmoglein 3 antibody [5H10] used in Western Blot (WB). GTX17502
Anti-Desmoglein 3 antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17502
Anti-Desmoglein 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX17502
Anti-Desmoglein 3 antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17502

Cat. No. GTX17502

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($419)
See all Desmoglein 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:200
IP 1:10 - 1:500
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The extracellular domain of human Desmoglein 3 [Swiss-Prot# P32926]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

desmoglein 3 , CDHF6 , PVA

Cellular Localization

Cell membrane

Background

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Desmoglein 3 antibody [5H10] used in Western Blot (WB). GTX17502

GTX17502 WB Image

WB analysis of HaCaT cell lysate using GTX17502 Desmoglein 3 antibody [5H10].

Anti-Desmoglein 3 antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17502

GTX17502 ICC/IF Image

ICC/IF analysis of HNSCC cells using GTX17502 Desmoglein 3 antibody [5H10].
Fixation : 1% formaldehyde
Permibilization : 0.1% Triton X-100

Anti-Desmoglein 3 antibody [5H10] used in IHC (Paraffin sections) (IHC-P). GTX17502

GTX17502 IHC-P Image

IHC-P analysis of human skin carcinoma tissue using GTX17502 Desmoglein 3 antibody [5H10].

Anti-Desmoglein 3 antibody [5H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17502

GTX17502 ICC/IF Image

ICC/IF analysis of A431 cells using GTX17502 Desmoglein 3 antibody [5H10].
Green : primary antibody
Blue : DAPI

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419