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Dopamine Receptor D2 antibody [HL1584]

Recombinant antibody
Anti-Dopamine Receptor D2 antibody [HL1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in Western Blot (WB). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063
Anti-Dopamine Receptor D2 antibody [HL1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637063

Cat. No. GTX637063

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1584

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Dopamine Receptor D2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Dopamine Receptor D2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dopamine receptor D2 , D2DR , D2R

Cellular Localization

Cell membrane

Background

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Dopamine Receptor D2 antibody [HL1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637063

GTX637063 ICC/IF Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in Western Blot (WB). GTX637063

GTX637063 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:100.
Red: MAP2, a neural marker, stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:100.
Red: MAP2, a neural marker, stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in IHC (Paraffin sections) (IHC-P). GTX637063

GTX637063 IHC-P Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Receptor D2 antibody [HL1584] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637063

GTX637063 ICC/IF Image

Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (GTX637063) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations