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Dopamine Transporter antibody

Orthogonal
Anti-Dopamine Transporter antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133152
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152
Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152
Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

Cat. No. GTX133152

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Dopamine Transporter products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

rat olfactory bulb

Predict Reactivity

Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Dopamine Transporter. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886846

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 , DAT , Dat1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

Database

Research Area

DATA IMAGES

Anti-Dopamine Transporter antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133152

GTX133152 ICC/IF Image

Dopamine Transporter antibody detects Dopamine Transporter protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152

GTX133152 IHC-P Image

Dopamine Transporter antibody detects Dopamine Transporter protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse olfactory bulb.
Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Transporter antibody used in IHC (Paraffin sections) (IHC-P). GTX133152

GTX133152 IHC-P Image

Dopamine Transporter antibody detects Dopamine Transporter protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse olfactory bulb.
Dopamine Transporter stained by Dopamine Transporter antibody (GTX133152) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152

GTX133152 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dopamine Transporter antibody (GTX133152) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Akiyama T et al. Stem Cells Transl Med 2021; 10 (4) : 572-581 Synthetic mRNA-based differentiation method enables early detection of Parkinson's phenotypes in neurons derived from Gaucher disease-induced pluripotent stem cells.
Application : ICC/IF
Submit a Reference

REVIEW

Dopamine Transporter antibody Cat. No. GTX133152

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Dopamine Transporter antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133152
Date : Anonymous submitted on 18-Apr-2023
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse primary neuron (Red)
Blocking : 5% BSA in 0.3% TritonX-PBS (-), 25°C, 0.5Hr
Fixation : 4% PFA
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-Dopamine Transporter antibody used in Western Blot (WB). GTX133152
Date : Anonymous submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : IMR-32 Neuroblast (ATCC CCL-127)
Amount used : 35 μg
Blocking : 5% BSA in PBST, 25°C, 1Hr
Primary Antibody : 2.22μg/ml, 4°C, 16Hr
Lane Description :
Lane 1 : IMR-32 (ATCC CCL-127) as control.
Lane 2-4 : IMR-32 (ATCC CCL-127) were differentiated and harvested on different days.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169