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Dynein antibody [74.1]

Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684
Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684
Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684
Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684
Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684
Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684
Anti-Dynein antibody [74.1] used in IHC (Whole mount) (IHC-Wm). GTX80684

Cat. No. GTX80684

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 74.1

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm, FACS, IP

Reactivity

 Human, Rat
Package
100 μg ($399)
See all Dynein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 2-4 μg/ml
IHC-Wm Assay dependent
FACS 1-2 μg/test
IP Assay dependent
Not tested in other applications.

Product Note

This antibody detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified bovine brain cytoplasmic dynein.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_625459

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynein light chain LC8-type 1 , dynein light chain LC8-type 2 , CDLC2 , DNCL1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus,Mitochondrion

Background

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.

Database

Research Area

DATA IMAGES

Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684

GTX80684 ICC/IF Image

ICC/IF analysis of C6 cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684

GTX80684 FACS Image

FACS analysis of C6 cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684

GTX80684 FACS Image

FACS analysis of U87-MG cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684

GTX80684 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Dynein antibody [74.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80684

GTX80684 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX80684 Dynein antibody [74.1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Dynein antibody [74.1] used in Flow cytometry (FACS). GTX80684

GTX80684 FACS Image

FACS analysis of Hela cells using GTX80684 Dynein antibody [74.1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Dynein antibody [74.1] used in IHC (Whole mount) (IHC-Wm). GTX80684

GTX80684 IHC-Wm Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

REFERENCE

Application Reference

Huang FI et al. Front Oncol 2019; 9 : 249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399