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E-Cadherin antibody [CDH1/2208R]

Anti-E-Cadherin antibody [CDH1/2208R] used in Flow cytometry (FACS). GTX02618
Anti-E-Cadherin antibody [CDH1/2208R] used in Western Blot (WB). GTX02618
Anti-E-Cadherin antibody [CDH1/2208R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02618
Anti-E-Cadherin antibody [CDH1/2208R] used in IHC (Paraffin sections) (IHC-P). GTX02618

Cat. No. GTX02618

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 CDH1/2208R

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all E-Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Observed MW

120-80, 135 kDa.

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human E-Cadherin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane , Endosome

Background

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody [CDH1/2208R] used in Flow cytometry (FACS). GTX02618

GTX02618 FACS Image

FACS analysis of MCF-7 cells using GTX02618 E-Cadherin antibody [CDH1/2208R].
Blue : Primary antibody
Red : Isotype control

Anti-E-Cadherin antibody [CDH1/2208R] used in Western Blot (WB). GTX02618

GTX02618 WB Image

WB analysis of MCF-7 whole cell lysate using GTX02618 E-Cadherin antibody [CDH1/2208R].

Anti-E-Cadherin antibody [CDH1/2208R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02618

GTX02618 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX02618 E-Cadherin antibody [CDH1/2208R].
Green : Primary antibody
Red : nuclear counterstain

Anti-E-Cadherin antibody [CDH1/2208R] used in IHC (Paraffin sections) (IHC-P). GTX02618

GTX02618 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02618 E-Cadherin antibody [CDH1/2208R].

REFERENCE

Application Reference

Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
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REVIEW

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Package List Price ($)
$ 529