APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:50-1:200 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888130
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
Cellular Localization
Cell junction , Cell membrane
Background
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692](/templates/default/js/lightbox/images/loading.gif "Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692")
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GTX629692 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [GT311] (GTX629692) diluted at 1:2000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629692 FACS Image
E-Cadherin antibody [GT311] (GTX629692) detects E-Cadherin protein by flow cytometry analysis.
Sample: MCF-7 cell.
Black: Unlabelled sample was used as a control.
Red: E-Cadherin antibody [GT311] (GTX629692) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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GTX629692 IP Image
E-adherin antibody [GT311] immunoprecipitates E-adherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. 30 μg MCF-7 cell whole cell extract
B. Control with 3 μg of preimmune Mouse IgG
C. Immunoprecipitation of E-adherin protein by 3 μg E-adherin antibody [GT311] (GTX629692)
5 % SDS-PAGE
The immunoprecipitated E-adherin protein was detected by E-adherin antibody [GT311] (GTX629692) diluted at 1:500.
[EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
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GTX629692 WB Image
E-cadherin antibody [GT311] detects E-cadherin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
5 % SDS-PAGE
E-cadherin antibody [GT311] (GTX629692) dilution: 1:1000
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REFERENCE
There are currently no references for E-Cadherin antibody [GT311] (GTX629692). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for E-Cadherin antibody [GT311] (GTX629692). Be the first to share your experience with this product.
![Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692](/upload/website/prouct_img/small/GTX629692/GTX629692_41400_20170623_WB_shRNA_watermark_w_23061202_222.webp "Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692](/upload/website/prouct_img/small/GTX629692/GTX629692_41400_20181207_FACS_w_23061202_932.webp "Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692](/upload/website/prouct_img/small/GTX629692/GTX629692_41400_IP_w_23061202_136.webp "Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692](/upload/website/prouct_img/small/GTX629692/GTX629692_41400_WB_w_23061202_475.webp "Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692](/upload/website/prouct_img/normal/GTX629692/GTX629692_41400_20170623_WB_shRNA_watermark_w_23061202_222.webp "Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692](/upload/website/prouct_img/normal/GTX629692/GTX629692_41400_20181207_FACS_w_23061202_932.webp "Anti-E-Cadherin antibody [GT311] used in Flow cytometry (FACS). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692](/upload/website/prouct_img/normal/GTX629692/GTX629692_41400_IP_w_23061202_136.webp "Anti-E-Cadherin antibody [GT311] used in Immunoprecipitation (IP). GTX629692")
![Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692](/upload/website/prouct_img/normal/GTX629692/GTX629692_41400_WB_w_23061202_475.webp "Anti-E-Cadherin antibody [GT311] used in Western Blot (WB). GTX629692")