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E2F3 antibody

Anti-E2F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31589
Anti-E2F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31589
Anti-E2F3 antibody used in Western Blot (WB). GTX31589

Cat. No. GTX31589

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all E2F3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , Human Lymph node Tissue Lysate(GTX29055)

Product Note

At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.The immunogen is located within amino acids 150 - 200 of E2F3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E2F transcription factor 3 , E2F-3

Cellular Localization

Nucleus

Background

This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Database

Research Area

DATA IMAGES

Anti-E2F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31589

GTX31589 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31589 E2F3 antibody.
Working concentration : 5 μg/ml

Anti-E2F3 antibody used in IHC (Paraffin sections) (IHC-P). GTX31589

GTX31589 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX31589 E2F3 antibody.
Working concentration : 20 μg/ml

Anti-E2F3 antibody used in Western Blot (WB). GTX31589

GTX31589 WB Image

WB analysis of human lymph node tissue lysate using GTX31589 E2F3 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399