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EAAT2 antibody

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062
Anti-EAAT2 antibody used in Western Blot (WB). GTX134062
Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

Cat. No. GTX134062

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all EAAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse EAAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887196

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 1 (glial high affinity glutamate transporter), member 2 , 1700091C19Rik , 2900019G14Rik , AI159670 , Eaat2 , GLT-1 , GLT1 , MGLT1

Cellular Localization

Membrane

Background

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Database

Research Area

DATA IMAGES

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

GTX134062 IHC-P Image

EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EAAT2 antibody used in Western Blot (WB). GTX134062

GTX134062 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EAAT2 antibody (GTX134062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EAAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134062

GTX134062 IHC-P Image

EAAT2 antibody detects EAAT2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: EAAT2 stained by EAAT2 antibody (GTX134062) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169