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EBI2 antibody

Anti-EBI2 antibody used in Western Blot (WB). GTX129712

Cat. No. GTX129712

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EBI2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

eGFP-human GPR183-transfected 293T cells(unboiled)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human EBI2 (GPR183). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886077

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor 183 , EBI2 , hEBI2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EBI2 antibody used in Western Blot (WB). GTX129712

GTX129712 WB Image

EBI2 (GPR183) antibody detects EBI2 (GPR183) protein by western blot analysis. Non-transfected (-) and -transfected (+, including eGFP-tag) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EBI2 (GPR183) antibody (GTX129712) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169