APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT5E7 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PA2G6 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proliferation-associated 2G4 , EBP1 , HG41 , PA2G4 , p382G4 , proliferationassociated 2G4
Cellular Localization
Cytoplasm,Nucleus,Nucleolus
Background
This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679](/templates/default/js/lightbox/images/loading.gif "Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679")
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GTX57679 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57679 EBP1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57679 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57679 EBP1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57679 FACS Image
FACS analysis of HeLa cells using GTX57679 EBP1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57679 WB Image
WB analysis of various samples using GTX57679 EBP1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : 293T whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : Jurkat whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
There are currently no references for EBP1 antibody [AT5E7] (GTX57679). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EBP1 antibody [AT5E7] (GTX57679). Be the first to share your experience with this product.
![Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679](/upload/website/prouct_img/small/GTX57679/GTX57679_20200204_IF2_w_23061123_218.webp "Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679](/upload/website/prouct_img/small/GTX57679/GTX57679_20200204_IF1_w_23061123_668.webp "Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Flow cytometry (FACS). GTX57679](/upload/website/prouct_img/small/GTX57679/GTX57679_20200204_FC_w_23061123_835.webp "Anti-EBP1 antibody [AT5E7] used in Flow cytometry (FACS). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Western Blot (WB). GTX57679](/upload/website/prouct_img/small/GTX57679/GTX57679_20200204_WB_w_23061123_412.webp "Anti-EBP1 antibody [AT5E7] used in Western Blot (WB). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679](/upload/website/prouct_img/normal/GTX57679/GTX57679_20200204_IF2_w_23061123_218.webp "Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679](/upload/website/prouct_img/normal/GTX57679/GTX57679_20200204_IF1_w_23061123_668.webp "Anti-EBP1 antibody [AT5E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Flow cytometry (FACS). GTX57679](/upload/website/prouct_img/normal/GTX57679/GTX57679_20200204_FC_w_23061123_835.webp "Anti-EBP1 antibody [AT5E7] used in Flow cytometry (FACS). GTX57679")
![Anti-EBP1 antibody [AT5E7] used in Western Blot (WB). GTX57679](/upload/website/prouct_img/normal/GTX57679/GTX57679_20200204_WB_w_23061123_412.webp "Anti-EBP1 antibody [AT5E7] used in Western Blot (WB). GTX57679")