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ECH1 antibody

KO/KD
Anti-ECH1 antibody used in Western Blot (WB). GTX114374
Anti-ECH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114374
Anti-ECH1 antibody used in Western Blot (WB). GTX114374
Anti-ECH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114374
Anti-ECH1 antibody used in Western Blot (WB). GTX114374

Cat. No. GTX114374

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ECH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Mouse brain , A549 , 293T

Predict Reactivity

Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA hydratase 1 , HPXEL

Cellular Localization

Mitochondrion , Peroxisome

Background

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ECH1 antibody used in Western Blot (WB). GTX114374

GTX114374 WB Image

KO/KD

Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody (GTX114374) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ECH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114374

GTX114374 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ECH1(GTX114374) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-ECH1 antibody used in Western Blot (WB). GTX114374

GTX114374 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114374 diluted at 1:1000

Anti-ECH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114374

GTX114374 IHC-P Image

ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
ECH1 antibody (GTX114374) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ECH1 antibody used in Western Blot (WB). GTX114374

GTX114374 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
ECH1 antibody
GTX114374 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169