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ECM29 antibody

Anti-ECM29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82906
Anti-ECM29 antibody used in Western Blot (WB). GTX82906
Anti-ECM29 antibody used in Western Blot (WB). GTX82906

Cat. No. GTX82906

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ECM29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
ICC/IF 1:250
IHC Assay dependent
Not tested in other applications.

Calculated MW

204 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_625878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ecm29 proteasome adaptor and scaffold , ECM29 , KIAA0368

Cellular Localization

Endoplasmic reticulum,Endosome,Nucleus,Cytoplasmic vesicle

Background

Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.

Database

Research Area

DATA IMAGES

Anti-ECM29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82906

GTX82906 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82906 ECM29 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-ECM29 antibody used in Western Blot (WB). GTX82906

GTX82906 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HeLa (Lane 1), K-562 (Lane 2) and NTERA-2 cl.D1 (Lane 3) using GTX82906 ECM29 antibody.
Dilution : 1:250

Anti-ECM29 antibody used in Western Blot (WB). GTX82906

GTX82906 WB Image

WB analysis of HeLa cells using GTX82906 ECM29 antibody.

REFERENCE

Application Reference

Kimura H et al. Cell Cycle 2014; 13 (12) : 1928-37 Centrosomes at M phase act as a scaffold for the accumulation of intracellular ubiquitinated proteins.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399