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EDIL3 antibody

Overexpression
Anti-EDIL3 antibody used in Western Blot (WB). GTX104086
Anti-EDIL3 antibody used in Western Blot (WB). GTX104086
Anti-EDIL3 antibody used in Western Blot (WB). GTX104086
Anti-EDIL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104086

Cat. No. GTX104086

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EDIL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Mouse brain , U87-MG conditioned medium , EDIL3-transfected 293T

Predict Reactivity

Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human EDIL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF like repeats and discoidin domains 3 , DEL1

Cellular Localization

Secreted

Background

The protein encoded by this gene is an integrin ligand. It plays an important role in mediating angiogenesis and may be important in vessel wall remodeling and development. It also influences endothelial cell behavior. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-EDIL3 antibody used in Western Blot (WB). GTX104086

GTX104086 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EDIL3 antibody (GTX104086) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EDIL3 antibody used in Western Blot (WB). GTX104086

GTX104086 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EDIL3 antibody (GTX104086) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EDIL3 antibody used in Western Blot (WB). GTX104086

GTX104086 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104086 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-EDIL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104086

GTX104086 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EDIL3(GTX104086) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169