APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for EGFR E746-A750del mutant protein. It does not recognize EGFR wide type protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen used to generate this antibody corresponds to human EGFR (E746-A750del).
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888572
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
epidermal growth factor receptor , ERBB , ERBB1 , HER1 , NISBD2 , PIG61 , mENA
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Nucleus
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Database
Research Area
DATA IMAGES
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GTX635716 WB Image
Various whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (E746-A750del) antibody [HL363] (GTX635716) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PC-9 cells express EGFR (Glu746-Ala750 deletion) protein and H1299 cells express wide type EGFR protein. (refer to PMID: 22157369)
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REFERENCE
There are currently no references for EGFR (E746-A750del) antibody [HL363] (GTX635716). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EGFR (E746-A750del) antibody [HL363] (GTX635716). Be the first to share your experience with this product.