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EHD1 antibody, Internal

Anti-EHD1 antibody, Internal used in Western Blot (WB). GTX49037
Anti-EHD1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX49037

Cat. No. GTX49037

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all EHD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human EHD1

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EH domain containing 1 , H-PAST , HPAST1 , PAST , PAST1

Cellular Localization

Cell membrane

Background

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-EHD1 antibody, Internal used in Western Blot (WB). GTX49037

GTX49037 WB Image

WB analysis of A549 cells using GTX49037 EHD1 antibody at 0.2-1μg/ml.

Anti-EHD1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX49037

GTX49037 IHC-P Image

IHC-P analysis of human heart tissue using GTX49037 EHD1 antibody at 1:100. Left to right : DAPI, EHD1, Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399