APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.3μg/ml |
5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89668-PEP).
Product Note
This antibody is expected to recognise EHD1 protein as well as EHD2.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EH domain containing 2 , PAST2
Cellular Localization
Cell membrane , Endosome membrane , Cytoplasm, cytosol
Background
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89668 WB Image
WB analysis of human placenta lysate using GTX89668 EHD2 antibody, C-term. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
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GTX89668 IHC-P Image
IHC-P analysis of human vessel using GTX89668 EHD2 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
There are currently no references for EHD2 antibody, C-term (GTX89668). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EHD2 antibody, C-term (GTX89668). Be the first to share your experience with this product.