GeneTex

  • Country / Location Selection

United States (US)

EIF2C2 antibody

Anti-EIF2C2 antibody used in Western Blot (WB). GTX131422
Anti-EIF2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131422
Anti-EIF2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX131422

Cat. No. GTX131422

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all EIF2C2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse, Zebrafish, Rabbit, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human EIF2C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886481

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

argonaute RISC catalytic component 2 , CASC7 , EIF2C2 , LINC00980 , PPD , Q10

Cellular Localization

Cytoplasm , P-body , Nucleus

Background

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-EIF2C2 antibody used in Western Blot (WB). GTX131422

GTX131422 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGO2 antibody (GTX131422) diluted at 1:1000.

Anti-EIF2C2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131422

GTX131422 ICC/IF Image

AGO2 antibody detects AGO2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AGO2 protein stained by AGO2 antibody (GTX131422) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-EIF2C2 antibody used in IHC (Paraffin sections) (IHC-P). GTX131422

GTX131422 IHC-P Image

EIF2C2 antibody detects EIF2C2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
EIF2C2 antibody (GTX131422) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jie-Ning Li et al. Front Cell Dev Biol 2021; 9 : 671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for EIF2C2 antibody (GTX131422). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169