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EIF3A antibody [N1N2], N-term

Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895
Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895
Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895
Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895

Cat. No. GTX118895

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all EIF3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

167 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human EIF3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 3 subunit A , EIF3 , EIF3S10 , P167 , TIF32 , eIF3-p170 , eIF3-theta , p180 , p185

Cellular Localization

Cytoplasm

Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Database

Research Area

DATA IMAGES

Anti-EIF3A antibody [N1N2], N-term used in Western Blot (WB). GTX118895

GTX118895 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
GTX118895 diluted at 1:500

Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895

GTX118895 ICC/IF Image

EIF3A antibody [N1N2], N-term detects EIF3A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EIF3A protein stained by EIF3A antibody [N1N2], N-term (GTX118895) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-EIF3A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118895

GTX118895 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using EIF3A(GTX118895) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EIF3A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118895

GTX118895 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using EIF3A(GTX118895) antibody at 1:200 dilution.

REFERENCE

Application Reference

Wang Q et al. Virus Res 2012; 167 (2) : 302-9 Regulation of hepatitis C virus translation initiation by iron: role of eIF3 and La protein.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169