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EMG1 antibody [1B8]

Overexpression
Anti-EMG1 antibody [1B8] used in Flow cytometry (FACS). GTX84576
Anti-EMG1 antibody [1B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84576
Anti-EMG1 antibody [1B8] used in Flow cytometry (FACS). GTX84576
Anti-EMG1 antibody [1B8] used in Western Blot (WB). GTX84576
Anti-EMG1 antibody [1B8] used in Western Blot (WB). GTX84576

Cat. No. GTX84576

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B8

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all EMG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EMG1 N1-specific pseudouridine methyltransferase , C2F , Grcc2f , NEP1

Cellular Localization

Nucleolus

Background

Involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

Database

DATA IMAGES

Anti-EMG1 antibody [1B8] used in Flow cytometry (FACS). GTX84576

GTX84576 FACS Image

FACS analysis of Jurkat cells using GTX84576 EMG1 antibody [1B8].
Red : Primary antibody
Blue : Negative control antibody

Anti-EMG1 antibody [1B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84576

GTX84576 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with EMG1 plasmid using GTX84576 EMG1 antibody [1B8].

Anti-EMG1 antibody [1B8] used in Flow cytometry (FACS). GTX84576

GTX84576 FACS Image

FACS analysis of HeLa cells using GTX84576 EMG1 antibody [1B8].
Red : Primary antibody
Blue : Negative control antibody

Anti-EMG1 antibody [1B8] used in Western Blot (WB). GTX84576

GTX84576 WB Image

Overexpression

WB analysis of HEK293T cells transfected with EMG1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84576 EMG1 antibody [1B8].
Loading : 5 ug per lane

Anti-EMG1 antibody [1B8] used in Western Blot (WB). GTX84576

GTX84576 WB Image

WB analysis of various cell lines using GTX84576 EMG1 antibody [1B8].
Loading : 35 ug per lane

REFERENCE

There are currently no references for EMG1 antibody [1B8] (GTX84576). Be the first to share your publications with this product.
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REVIEW

There are currently no reviews for EMG1 antibody [1B8] (GTX84576). Be the first to share your experience with this product.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519