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EMI1 antibody [EMI1/1176]

Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689
Anti-EMI1 antibody [EMI1/1176] used in Protein Array (Protein Array). GTX34689
Anti-EMI1 antibody [EMI1/1176] used in Western Blot (WB). GTX34689
Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

Cat. No. GTX34689

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EMI1/1176

Isotype

 IgG2a

Application

 WB, IHC-P, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all EMI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml for 60 minutes at RT
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EMI1 , FBX5 , FBXO5 , Fbox protein 5 , Fbxo31 , Emi1 , F-box protein 5

Cellular Localization

Nucleus,Cytoplasm,Cytoskeleton,Spindle

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

GTX34689 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34689 EMI1 antibody [EMI1/1176].

Anti-EMI1 antibody [EMI1/1176] used in Protein Array (Protein Array). GTX34689

GTX34689 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using EMI1 Mouse Monoclonal Antibody (EMI1/1176). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-EMI1 antibody [EMI1/1176] used in Western Blot (WB). GTX34689

GTX34689 WB Image

WB analysis of HeLa, HepG2, HEK293, and K562 cell lysates using GTX34689 EMI1 antibody [EMI1/1176].

Anti-EMI1 antibody [EMI1/1176] used in IHC (Paraffin sections) (IHC-P). GTX34689

GTX34689 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX34689 EMI1 antibody [EMI1/1176].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529