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EMR2 antibody [2A1]

Anti-EMR2 antibody [2A1] used in Flow cytometry (FACS). GTX31227

Cat. No. GTX31227

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A1

Isotype

 IgG1

Application

 WB, IHC-Fr, FACS, IP

Reactivity

 Human
Package
100 μg ($399)
See all EMR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr 1/50-1/200
FACS 1/50-1/200
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Product Note

Clone 2A1 specifically recognizes the stalk region of EMR2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adhesion G protein-coupled receptor E2 , CD312 , CD97 , EMR2 , VBU

Cellular Localization

Cell membrane

Background

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-EMR2 antibody [2A1] used in Flow cytometry (FACS). GTX31227

GTX31227 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX31227 EMR2 antibody [2A1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399