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ENO1 + ENO2 antibody [AT1G7]

Anti-ENO1 + ENO2 antibody [AT1G7] used in Western Blot (WB). GTX57673
Anti-ENO1 + ENO2 antibody [AT1G7] used in Flow cytometry (FACS). GTX57673
Anti-ENO1 + ENO2 antibody [AT1G7] used in Western Blot (WB). GTX57673
Anti-ENO1 + ENO2 antibody [AT1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57673

Cat. No. GTX57673

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1G7

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all ENO1 + ENO2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

Based on internal testing result, this antibody does not cross react with human ENO3.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1G7 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Alpha-enolase protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 1 , enolase 2 , ENO1L1 , HEL-S-17 , MPB1 , NNE , PPH , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Database

DATA IMAGES

Anti-ENO1 + ENO2 antibody [AT1G7] used in Western Blot (WB). GTX57673

GTX57673 WB Image

WB analysis of various samples using GTX57673 ENO1 + ENO2 antibody [AT1G7].
Lane 1 : PC-3 whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-ENO1 + ENO2 antibody [AT1G7] used in Flow cytometry (FACS). GTX57673

GTX57673 FACS Image

FACS analysis of LNCap cells using GTX57673 ENO1 + ENO2 antibody [AT1G7].
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-ENO1 + ENO2 antibody [AT1G7] used in Western Blot (WB). GTX57673

GTX57673 WB Image

WB analysis of various samples using GTX57673 ENO1 + ENO2 antibody [AT1G7].
Lane 1 : Recombinant Human ENO1 (Alpha-enolase) protein
Lane 2 : Recombinant Human ENO2 (Gamma-enolase) protein
Lane 3 : Recombinant Human ENO3 (Beta-enolase) protein
Loading : 50 ng
Dilution : 1:1000

Anti-ENO1 + ENO2 antibody [AT1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57673

GTX57673 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57673 ENO1 + ENO2 antibody [AT1G7].
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399