This is specific for p300 protein. This recognizes residues 1921-2023.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]
FACS (Intracellular staining) analysis of THP-1 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 1 μg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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GTX30618 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX30618 EP300 antibody [RW105]. Green : Primary antibody Blue : DAPI Permeabilization : 0.05% Triton X-100 in PBS Dilution : 5 μg/mL
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GTX30618 FACS Image
FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 2 μg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
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GTX30618 WB Image
WB analysis of HeLa nuclear extract using GTX30618 EP300 antibody [RW105]. Dilution : 1:250