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EP300 antibody

Comparison Orthogonal
Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238
Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238
Anti-EP300 antibody used in Western Blot (WB). GTX134238

Cat. No. GTX134238

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EP300 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

264 kDa. ( Note )

Positive Control

A431 nuclear extract

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EP300. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887243

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

E1A binding protein p300 , KAT3B , MKHK2 , RSTS2 , p300

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238

GTX134238 IHC-P Image

EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EP300 antibody used in IHC (Paraffin sections) (IHC-P). GTX134238

GTX134238 IHC-P Image

EP300 antibody detects EP300 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
EP300 stained by EP300 antibody (GTX134238) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-EP300 antibody used in Western Blot (WB). GTX134238

GTX134238 WB Image

Comparison Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EP300 antibody (GTX134238) diluted at 1:500 and competitor's antibody (sc-584) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169