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EPO antibody [4F11]

Anti-EPO antibody [4F11] used in Western Blot (WB). GTX82784
Anti-EPO antibody [4F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82784

Cat. No. GTX82784

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F11

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all EPO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human EPO expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erythropoietin , DBAL , ECYT5 , EP , MVCD2

Cellular Localization

Secreted

Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-EPO antibody [4F11] used in Western Blot (WB). GTX82784

GTX82784 WB Image

WB analysis of HEK293 (1) and EPO(AA: 28-193)-hIgGFc transfected HEK293 (2) cell lysate using GTX82784 EPO antibody [4F11].

Anti-EPO antibody [4F11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82784

GTX82784 ICC/IF Image

ICC/IF analysis of GC7901 cells using GTX82784 EPO antibody [4F11].
Green : EPO
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399