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ERK1/2 antibody [3F8]

Anti-ERK1/2 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83006
Anti-ERK1/2 antibody [3F8] used in Western Blot (WB). GTX83006
Anti-ERK1/2 antibody [3F8] used in Flow cytometry (FACS). GTX83006

Cat. No. GTX83006

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F8

Isotype

 IgG2b

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ERK1/2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ERK1/2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-ERK1/2 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83006

GTX83006 IHC-P Image

IHC-P analysis of human Liver tissue using GTX83006 ERK1/2 antibody [3F8].

Anti-ERK1/2 antibody [3F8] used in Western Blot (WB). GTX83006

GTX83006 WB Image

WB analysis of Jurkat (1), HeLa (2), A431 (3) and NIH3T3 (4) cell lysate using GTX83006 ERK1/2 antibody [3F8].

Anti-ERK1/2 antibody [3F8] used in Flow cytometry (FACS). GTX83006

GTX83006 FACS Image

FACS analysis of Jurkat cells using GTX83006 ERK1/2 antibody [3F8].
Green : ERK1/2
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399