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ERK1/2 antibody

Orthogonal
Anti-ERK1/2 antibody used in Western Blot (WB). GTX50560
Anti-ERK1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50560

Cat. No. GTX50560

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ERK1/2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 200~204 (F-L-T-E-Y) derived from human ERK1/2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-ERK1/2 antibody used in Western Blot (WB). GTX50560

GTX50560 WB Image

Orthogonal

WB analysis of extracts from HeLa and 293 cells using GTX50560 ERK1/2 antibody.

Anti-ERK1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50560

GTX50560 IHC-P Image

Orthogonal

IHC-P analysis of human colorectal tissue using GTX50560 ERK1/2 antibody.

REFERENCE

Application Reference

M Rokni et al. Iran J Allergy Asthma Immunol 2024; Interferon-γ Induces Interleukin-6 Production and Alpha-smooth Muscle Actin Expression in Systemic Sclerosis Fibroblasts
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub) : 142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399