APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 2 μg/ml |
| 1:100 |
| 1-3 μl |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody does not cross react with ERK2.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full-length human recombinant protein expressed in bacteria.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780](/templates/default/js/lightbox/images/loading.gif "Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780")
|
GTX15780 ICC/IF Image
ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
|
|
|
GTX15780 IHC-P Image
IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95ºC
|
|
|
GTX15780 ICC/IF Image
ICC/IF analysis of untreated and siRNA knockdown treated HeLa cells using GTX15780 ERK1 antibody [12D11].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 for at least 1 hour at room temperature
|
|
|
GTX15780 ChIP assay Image
ChIP analysis of 1x10⁶ HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes using GTX15780 ERK1 antibody [12D11]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 of Egr1.
IP reaction : 1.0ul antibody / 100ul sample
|
|
|
GTX15780 WB Image
WB analysis of 25 ug of various whole cell lysates using GTX15780 ERK1 antibody [12D11].
Dilution : 1:1000
|
REFERENCE
There are currently no references for ERK1 antibody [12D11] (GTX15780). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ERK1 antibody [12D11] (GTX15780). Be the first to share your experience with this product.
![Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780](/upload/website/prouct_img/small/GTX15780/GTX15780_311_ICC-IF_w_23060620_404.webp "Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780")
![Anti-ERK1 antibody [12D11] used in IHC (Paraffin sections) (IHC-P). GTX15780](/upload/website/prouct_img/small/GTX15780/GTX15780_1040_IHC-P_w_23060620_259.webp "Anti-ERK1 antibody [12D11] used in IHC (Paraffin sections) (IHC-P). GTX15780")
![Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780](/upload/website/prouct_img/small/GTX15780/GTX15780_312_ICC-IF_w_23060620_855.webp "Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780")
![Anti-ERK1 antibody [12D11] used in ChIP assay (ChIP assay). GTX15780](/upload/website/prouct_img/small/GTX15780/GTX15780_5_ChIP_w_23060620_186.webp "Anti-ERK1 antibody [12D11] used in ChIP assay (ChIP assay). GTX15780")
![Anti-ERK1 antibody [12D11] used in Western Blot (WB). GTX15780](/upload/website/prouct_img/small/GTX15780/GTX15780_1534_WB_w_23060620_378.webp "Anti-ERK1 antibody [12D11] used in Western Blot (WB). GTX15780")
![Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780](/upload/website/prouct_img/normal/GTX15780/GTX15780_311_ICC-IF_w_23060620_404.webp "Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780")
![Anti-ERK1 antibody [12D11] used in IHC (Paraffin sections) (IHC-P). GTX15780](/upload/website/prouct_img/normal/GTX15780/GTX15780_1040_IHC-P_w_23060620_259.webp "Anti-ERK1 antibody [12D11] used in IHC (Paraffin sections) (IHC-P). GTX15780")
![Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780](/upload/website/prouct_img/normal/GTX15780/GTX15780_312_ICC-IF_w_23060620_855.webp "Anti-ERK1 antibody [12D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15780")
![Anti-ERK1 antibody [12D11] used in ChIP assay (ChIP assay). GTX15780](/upload/website/prouct_img/normal/GTX15780/GTX15780_5_ChIP_w_23060620_186.webp "Anti-ERK1 antibody [12D11] used in ChIP assay (ChIP assay). GTX15780")
![Anti-ERK1 antibody [12D11] used in Western Blot (WB). GTX15780](/upload/website/prouct_img/normal/GTX15780/GTX15780_1534_WB_w_23060620_378.webp "Anti-ERK1 antibody [12D11] used in Western Blot (WB). GTX15780")