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ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [MAPK-YT]

Cat. No. GTX01452

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MAPK-YT

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Yeast
Package
100 μg ($429)
See all ERK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
ICC/IF 1μg/ml
IHC-P 0.4-1μg/ml

Note :

IHC-P
Antigen retrieval by Heat
Not tested in other applications.

Product Note

Superfamily members of Mapk3 are not reactive to this antibody.

Predict Reactivity

Bovine, Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS, 1.2% Sodium acetate, 2mg BSA

Preservative

0.01mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen activated protein kinase 3 , mitogen activated protein kinase 1 , ERK1 , ERT2 , Erk-1 , Esrk1 , MAPK1 , MNK1 , Prkm3 , p44 , p44erk1 , p44mapk , ERK-2 , ERT1 , Erk2 , p42-MAPK

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

REFERENCE

Application Reference

Dom?nguez-Mart?nez DA et al. Viruses 2023; 15 (3) IL-8 Secreted by Gastric Epithelial Cells Infected with Helicobacter pylori CagA Positive Strains Is a Chemoattractant for Epstein-Barr Virus Infected B Lymphocytes.
Application : WB
Reactivity : Human

Clone Reference

Zhu Z et al. J Exp Clin Cancer Res. 2015; Phosphorylated AKT1 is associated with poor prognosis in esophageal squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Zhang Z et al. BMC Med. 2009; Phosphorylated ERK is a potential predictor of sensitivity to sorafenib when treating hepatocellular carcinoma: evidence from an in vitro study.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429