GeneTex

  • Country / Location Selection

United States (US)

ERO1L antibody

Anti-ERO1L antibody used in IHC (Paraffin sections) (IHC-P). GTX112589
Anti-ERO1L antibody used in Western Blot (WB). GTX112589
Anti-ERO1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112589
Anti-ERO1L antibody used in Western Blot (WB). GTX112589

Cat. No. GTX112589

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Chicken
Package
100 μl ($399),
25 μl ($169)
See all ERO1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse stomach , HCT116 , MCF-7

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERO1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endoplasmic reticulum oxidoreductase 1 alpha , ERO1-L , ERO1-L-alpha , ERO1-alpha , ERO1L , ERO1LA , Ero1alpha

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side

Background

Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Database

Research Area

DATA IMAGES

Anti-ERO1L antibody used in IHC (Paraffin sections) (IHC-P). GTX112589

GTX112589 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ERO1L(GTX112589) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERO1L antibody used in Western Blot (WB). GTX112589

GTX112589 WB Image

ERO1L antibody detects ERO1L protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract
B. 30 μg MCF-7 whole cell lysate/extract
10 % SDS-PAGE
ERO1L antibody (GTX112589) dilution: 1:1000

Anti-ERO1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112589

GTX112589 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using ERO1L(GTX112589) antibody at 1:500 dilution.

Anti-ERO1L antibody used in Western Blot (WB). GTX112589

GTX112589 WB Image

ERO1L antibody detects ERO1L protein by Western blot analysis.
A. 50 μg mouse stomach lysate/extract
10 % SDS-PAGE
ERO1L antibody (GTX112589) dilution: 1:1000

REFERENCE

Application Reference

Zilli F et al. EMBO Mol Med 2021; 13 (4) : e13162 The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells.
Application : IHC-P
Reactivity : Human
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Wang SH et al. Anim Biotechnol 2018; : 43117 Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.
Application : WB
Reactivity : Chicken
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ERO1L antibody (GTX112589). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169