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EYA3 antibody, Internal

Anti-EYA3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX77877
Anti-EYA3 antibody, Internal used in Western Blot (WB). GTX77877

Cat. No. GTX77877

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all EYA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human EYA3

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_424825

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EYA transcriptional coactivator and phosphatase 3

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Database

DATA IMAGES

Anti-EYA3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX77877

GTX77877 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX77877 EYA3 antibody at 4.0-8.0μg/ml.

Anti-EYA3 antibody, Internal used in Western Blot (WB). GTX77877

GTX77877 WB Image

WB analysis of HepG2 cells using GTX77877 EYA3 antibody at 0.05-1.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399