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Edc3 antibody [N2C2], Internal

Anti-Edc3 antibody [N2C2], Internal used in Western Blot (WB). GTX116386
Anti-Edc3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116386
Anti-Edc3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116386
Anti-Edc3 antibody [N2C2], Internal used in Western Blot (WB). GTX116386

Cat. No. GTX116386

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Edc3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

NIH-3T3 , C2C12 , A431

Predict Reactivity

Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Edc3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624271

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enhancer of mRNA decapping 3 , LSM16 , MRT50 , YJDC , YJEFN2 , hYjeF_N2-15q23

Cellular Localization

Cytoplasm , P-body

Background

EDC3 is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Edc3 antibody [N2C2], Internal used in Western Blot (WB). GTX116386

GTX116386 WB Image

Edc3 antibody [N2C2], Internal detects EDC3 protein by Western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Edc3 antibody [N2C2], Internal (GTX116386) dilution: 1:1000

Anti-Edc3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116386

GTX116386 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Edc3(GTX116386) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Edc3 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116386

GTX116386 IHC-P Image

Edc3 antibody [N2C2], Internal detects Edc3 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Edc3 antibody [N2C2], Internal (GTX116386) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Edc3 antibody [N2C2], Internal used in Western Blot (WB). GTX116386

GTX116386 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX116386 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169