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Emerin antibody

KO/KD
Anti-Emerin antibody used in Western Blot (WB). GTX113427
Anti-Emerin antibody used in Western Blot (WB). GTX113427
Anti-Emerin antibody used in IHC (Paraffin sections) (IHC-P). GTX113427
Anti-Emerin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113427

Cat. No. GTX113427

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Emerin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa nucleus , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Emerin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165701

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

emerin , EDMD , LEMD5 , STA

Cellular Localization

Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Emerin antibody used in Western Blot (WB). GTX113427

GTX113427 WB Image

KO/KD

Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody (GTX113427) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Emerin antibody used in Western Blot (WB). GTX113427

GTX113427 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
12% SDS PAGE
GTX113427 diluted at 1:5000

Anti-Emerin antibody used in IHC (Paraffin sections) (IHC-P). GTX113427

GTX113427 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Emerin(GTX113427) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Emerin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113427

GTX113427 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Emerin(GTX113427) antibody at 1:500 dilution.

REFERENCE

Application Reference

Abdou AG et al. J Immunoassay Immunochem 2018; 39 (5) : 521-537 Evaluation of the diagnostic value of emerin and CD56 in papillary thyroid carcinoma - an immunohistochemical study.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169