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Endothelin 1 antibody [C1C3]

Overexpression
Anti-Endothelin 1 antibody [C1C3] used in Western Blot (WB). GTX116033
Anti-Endothelin 1 antibody [C1C3] used in Western Blot (WB). GTX116033

Cat. No. GTX116033

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Endothelin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HUVEC , transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Endothelin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036854

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endothelin 1 , ARCND3 , ET1 , HDLCQ7 , PPET1 , QME

Cellular Localization

Secreted

Background

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Endothelin 1 antibody [C1C3] used in Western Blot (WB). GTX116033

GTX116033 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Endothelin 1 antibody [C1C3] (GTX116033) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Endothelin 1 antibody [C1C3] used in Western Blot (WB). GTX116033

GTX116033 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Endothelin 1 antibody [C1C3] (GTX116033) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Fathy A et al. Turk Patoloji Derg 2018; (Epub) EZH2, Endothelin-1, and CD34 as Biomarkers of Aggressive Cervical Squamous Cell Carcinoma: An Immunohistochemical Study.
Application : IHC-P
Reactivity : Human
Russignan A et al. Br J Haematol 2017; (Epub) Endothelin-1 receptor blockade as new possible therapeutic approach in multiple myeloma.
Application : WB
Reactivity : Human
Wang JW et al. Physiol Rep 2017; 5 (1) Endothelin-1 and ET receptors impair left ventricular function by mediated coronary arteries dysfunction in chronic intermittent hypoxia rats.
Paczkowska E et al. J Spinal Cord Med 2014; Evidence for proangiogenic cellular and humoral systemic response in patients with acute onset of spinal cord injury.
Application : ICC/IF
Lu JW et al. PLoS One 2014; 9 (1) : e85318 Overexpression of endothelin 1 triggers hepatocarcinogenesis in zebrafish and promotes cell proliferation and migration through the AKT pathway.
Application : IHC
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169