GeneTex

  • Country / Location Selection

United States (US)

Enterovirus 71 3D antibody [4]

Anti-Enterovirus 71 3D antibody [4] used in Western Blot (WB). GTX630193
Anti-Enterovirus 71 3D antibody [4] used in Western Blot (WB). GTX630193

Cat. No. GTX630193

Reference 11

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Coxsackievirus A6, Enterovirus 71
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus 71 3D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Positive Control

EV71 C2-infected RD , CVA6-infected RD

Product Note

This antibody is raised against Enterovirus 71, and it may cross react with Coxsackievirus A6 based on internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus 71

Immunogen

The immunogen used to generate this antibody corresponds to EV71 3D

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888196

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus 71 RNA-directed RNA polymerase , Enterovirus 71 Protein 3D , EV71 RNA-directed RNA polymerase , EV71 Protein 3D

Research Area

DATA IMAGES

Anti-Enterovirus 71 3D antibody [4] used in Western Blot (WB). GTX630193

GTX630193 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (20 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3D antibody [4] (GTX630193) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus 71 3D antibody [4] used in Western Blot (WB). GTX630193

GTX630193 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Enterovirus 71 3D antibody [4] (GTX630193) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang X et al. J Cell Biol 2023; 222 (12) Inhibition of lysosome-tethered Ragulator-Rag-3D complex restricts the replication of Enterovirus 71 and Coxsackie A16.
Yu J et al. Virulence 2022; 13 (1) : 241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.
Application : WB
Zhang Q et al. Virol Sin 2021; (Epub) ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles.
Kuo RL et al. RNA Biol 2021; : 44209 Molecular determinants and heterogeneity underlying host response to EV-A71 infection at single-cell resolution.
Application : WB
Lin JY et al. Sci Rep 2020; 10 (1) : 15234 Autophagy is induced and supports virus replication in Enterovirus A71-infected human primary neuronal cells.
Application : WB, ICC/IF
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Enterovirus 71 3D antibody [4] (GTX630193). Be the first to share your experience with this product.
Submit a Review
Application Reference
Wang X et al. J Cell Biol 2023; 222 (12) Inhibition of lysosome-tethered Ragulator-Rag-3D complex restricts the replication of Enterovirus 71 and Coxsackie A16.
Yu J et al. Virulence 2022; 13 (1):241-257 Regulation of host factor γ-H2AX level and location by enterovirus A71 for viral replication.
Application : WB
Zhang Q et al. Virol Sin 2021; (Epub) ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles.
Kuo RL et al. RNA Biol 2021;:44209 Molecular determinants and heterogeneity underlying host response to EV-A71 infection at single-cell resolution.
Application : WB
Lin JY et al. Sci Rep 2020; 10 (1):15234 Autophagy is induced and supports virus replication in Enterovirus A71-infected human primary neuronal cells.
Application : WB, ICC/IF
Sun J et al. Sci Rep 2020; 10 (1):8159 Drug repurposing of pyrimidine analogs as potent antiviral compounds against human enterovirus A71 infection with potential clinical applications.
Application : WB
Lee KM et al. RNA Biol 2020; 17 (4):608-622 The RNA-dependent RNA polymerase of enterovirus A71 associates with ribosomal proteins and positively regulates protein translation.
Application : WB, IP
Huang HI et al. Viruses 2019; 11 (12) EV71 Infection Induces IFN帣 Expression in Neural Cells.
Application : WB
Liao YT et al. Biomed Pharmacother 2019; 118:109271 Anti-inflammatory and antiviral effects of minocycline in enterovirus 71 infections.
Liou AT et al. Sci Rep. 2016; 6:31151 A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71.
Application : WB
Leong SY et al. PLoS Pathog 2015; 11 (3):e1004686 The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71.
Application : WB
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations