APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Sheep, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
epithelial cell adhesion molecule , DIAR5 , EGP-2 , EGP314 , EGP40 , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK-1 , TACSTD1 , TROP1
Background
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Database
Research Area
DATA IMAGES
![Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998](/templates/default/js/lightbox/images/loading.gif "Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998")
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GTX636998 FACS Image
EpCAM antibody [HL1524] (GTX636998) detects EpCAM protein by flow cytometry analysis.
Sample: HCT-116, HeLa and 4T1 cell.
Gray: Rabbit IgG Isotype control.
Red: EpCAM antibody [HL1524] (GTX636998) dilution: 1:50.
Acquisition of 50,000 (HCT-116 and HeLa cell) and 100,000 (4T1 cell) events were collected for flow cytometry analysis.
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GTX636998 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EpCAM antibody [HL1524] (GTX636998) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636998 IHC-P Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636998 IHC-P Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636998 IHC-P Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636998 IHC-P Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded HCT116 xenograft.
EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636998 ICC/IF Image
EpCAM antibody [HL1524] detects EpCAM protein at cell membrane and cell junction by immunofluorescent analysis.
Sample: HCT116 and HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EpCAM stained by EpCAM antibody [HL1524] (GTX636998) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for EpCAM antibody [HL1524] (GTX636998). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EpCAM antibody [HL1524] (GTX636998). Be the first to share your experience with this product.
![Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_44732_20220829_FACS_22090701_835.webp "Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998")
![Anti-EpCAM antibody [HL1524] used in Western Blot (WB). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_44732_20220708_WB_22071401_357.webp "Anti-EpCAM antibody [HL1524] used in Western Blot (WB). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_44732_20220715_IHC-P_M_22071823_418.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_44732_20220715_IHC-P_22071823_574.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_T-44683_20220610_IHC-P_M_22062121_751.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_T-44683_20220617_IHC-P_22062121_882.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636998](/upload/website/prouct_img/small/GTX636998/GTX636998_T-44683_20220624_ICC_IF_22062919_441.webp "Anti-EpCAM antibody [HL1524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636998")
![Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_44732_20220829_FACS_22090701_835.webp "Anti-EpCAM antibody [HL1524] used in Flow cytometry (FACS). GTX636998")
![Anti-EpCAM antibody [HL1524] used in Western Blot (WB). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_44732_20220708_WB_22071401_357.webp "Anti-EpCAM antibody [HL1524] used in Western Blot (WB). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_44732_20220715_IHC-P_M_22071823_418.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_44732_20220715_IHC-P_22071823_574.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_T-44683_20220610_IHC-P_M_22062121_751.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_T-44683_20220617_IHC-P_22062121_882.webp "Anti-EpCAM antibody [HL1524] used in IHC (Paraffin sections) (IHC-P). GTX636998")
![Anti-EpCAM antibody [HL1524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636998](/upload/website/prouct_img/normal/GTX636998/GTX636998_T-44683_20220624_ICC_IF_22062919_441.webp "Anti-EpCAM antibody [HL1524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636998")