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EpCAM antibody [VU-1D9]

Cat. No. GTX00606

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VU-1D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human
Package
100 μg ($339)
See all EpCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody recognizes an epitope within EGF-like domain I of CD326 / EpCAM.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Small cell lung carcinoma cell line H69.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2885203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIAR5 , EGP2 , EGP314 , EGP40 , EPCAM , ESA , HNPCC8 , KS1/4 , KSA , M4S1 , MIC18 , MK1 , TACSTD1 , TROP1 , epithelial cell adhesion molecule , EpCAM , CD326

Cellular Localization

Lateral cell membrane,Cell junction,Tight junction

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Database

Research Area

REFERENCE

Application Reference

Hideharu Mikami et al. bioRxiv 2020; (Epub) Virtual-freezing fluorescence imaging flow cytometry.
Application : FACS
Reactivity : Human

Clone Reference

Alecia-Jane Twigger et al. Nat Commun 2022; 13 (1):562 Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Hideharu Mikami et al. Nat Commun 2020; 11 (1):1162 Virtual-freezing fluorescence imaging flow cytometry
Application : FACS
Reactivity : Human
Yoshida M et al. Biotechnol Bioeng 2017; (Epub) Preferential capture of EpCAM-expressing extracellular vesicles on solid surfaces coated with an aptamer-conjugated zwitterionic polymer.
Lyberopoulou A et al. PLoS One 2015; 10 (4):e0123902 Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339